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KMID : 0370219860300060311
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Yakhak Hoeji
1986 Volume.30 No. 6 p.311 ~ p.316
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UV-HPLC Determination of Carboxyl Group Using 2-Bromoacetyltriphenylene as a Pre-labeling Reagent
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Abstract
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A new UV labeling reagent was developed and used in HPLC for the determination of prostaglandin E2 which have weak UV light-absorbing propertiy. This reagent, 2-bromoacetyltriphenylene, was synthesized by the bromination of 2-acetyltriphenylene which was obtained from
triphenylene by Friedel-Crafts reaction. The wave length maximum ()mac N) of this reagent was 268nm. Prostaglandin E2 was extracted from prostaglandin E2-13-cyclodextrin using a Sep-pak C18 cartridge. The prostaglandin E2 was labeled with 2-bromoacetyl-triphenylene in acetonitrile using 18-crown-6-ether as catalyst. Derivatized prostaglandins were separated on a reversed-phase column (Radial-pak) p-Bondapak Cis using acetonitrile: water=60 : 40 as mobile phase. The effluent was monitored by UV detector at 254nm filter kit. Linearity of calibration curve was obtained between 30ng and 140ng, and the lower limit of detection was 5ng.
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